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Published: 2016-07-27

Expression Pattern of Mouse Vasa Homologue (MVH) in the Ovaries of C57BL/6 Female Mice

Kunkun Song, Wenwen Ma, Cong Huang, Jiahui Ding, Dandan Cui, Mingmin Zhang

(Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan, Hubei, China (mainland))

Med Sci Monit 2016; 22:2656-2663

DOI: 10.12659/MSM.899830


BACKGROUND: Vasa (a DEAD-box helicase, also known as Ddx4) is an ATP-dependent RNA helicase highly conserved among all animals. Research on the presence and function of DDX4 in female mammals is limited. To gain greater insight into its distribution and role in female mice, we detected the expression of DDX4 protein in the ovaries and analyzed its expression pattern.
MATERIAL AND METHODS: MVH was detected in the cytoplasm of oocytes in all non-apoptotic follicles.
RESULTS: In the present study, we found that higher expression levels of ~55–60 kDa MVH isoform in the ovaries were followed by the accumulations of preovulatory follicles.
CONCLUSIONS: Higher levels of MVH protein in the ovaries might prepare oocytes for the competence to resume meiosis.

Keywords: Mice, Inbred C57BL, Ovarian Follicle, RecQ Helicases



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